Plant .ALPHA.-Glucosidase: Molecular Analysis of Rice .ALPHA.-Glucosidase and Degradation Mechanism of Starch Granules in Germination Stage
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- Nakai Hiroyuki
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- Ito Tatsuya
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- Tanizawa Shigeki
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- Matsubara Kazuki
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- Yamamoto Takeshi
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- Okuyama Masayuki
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- Mori Haruhide
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- Chiba Seiya
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- Sano Yoshio
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- Kimura Atsuo
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
Bibliographic Information
- Other Title
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- 植物α-グルコシダーゼ,特にイネ酵素の分子解析と発芽時の澱粉粒分解に関する研究
- Plant α-Glucosidase: Molecular Analysis of Rice α-Glucosidase and Degradation Mechanism of Starch Granules in Germination Stage
- Plant アルファ Glucosidase Molecular Analysis of Rice アルファ Glucosidase and Degradation Mechanism of Starch Granules in Germination Stage
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Abstract
In germination of plant seeds, storage starch is principally degraded by the combination of amylolytic enzymes. As starch is an insoluble granule, a conventional view of the degradation pathway is that the initial attack is performed by α-amylase having the starch granule-binding ability. Plant α-glucosidase was also capable of adsorbing and hydrolyzing starch granules directly, indicating a possible second pathway: the direct liberation of glucose from starch granules by plant α-glucosidase rather than the α-amylase-mediated system. We found that the starch-binding site of plant α-glucosidase was situated in its C-terminal region, of which function was independent of the catalytic domain. Site-directed mutagenesis analysis on the aromatic amino acid residues conserved in this region revealed that Trp803 and Phe895 of rice α-glucosidase were responsible for binding to starch granules. Mold α-glucosidases were devoid of the ability to attack starch granules. In plant seeds, multiple α-glucosidases have been observed. Two types of α-glucosidases, insoluble and soluble enzymes, were found in the germinating stage of rice. Expression patterns of their activities classified 14 rice varieties into two groups (Groups 1 and 2). In Group 1 varieties, insoluble enzyme decreased immediately after germination. The soluble enzyme increased by de novo synthesis. Group 2 maintained a constant activity level of insoluble and soluble α-glucosidases in germination. From Groups 1 and 2, we selected varieties of Akamai and Nipponbare, respectively, of which analysis elucidated interesting molecular mechanisms of insoluble and soluble enzymes: i) isoform and isozyme formations by post-translational proteolysis as well as by chromosomal gene expression; ii) characterization of purified enzymes exhibiting different activities to starch granules.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 53 (2), 137-142, 2006
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390282681270752128
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- NII Article ID
- 10016802840
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- NII Book ID
- AN10453916
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- COI
- 1:CAS:528:DC%2BD28Xmt12itLY%3D
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- ISSN
- 18807291
- 13447882
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- NDL BIB ID
- 7968737
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed