Starch properties of transgenic sweetpotato plants modified by RNA interference of the starch synthase 2 gene

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  • Kitahara Kanefumi
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
  • Takahata Yasuhiro
    National Agricultural Research Center for Kyushu Okinawa Region, National Agriculture and Food Research Organization
  • Otani Motoyasu
    Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University
  • Tanaka Masaru
    National Agricultural Research Center for Kyushu Okinawa Region, National Agriculture and Food Research Organization
  • Katayama Kenji
    National Agricultural Research Center for Kyushu Okinawa Region, National Agriculture and Food Research Organization
  • Yoshinaga Masaru
    National Agricultural Research Center for Kyushu Okinawa Region, National Agriculture and Food Research Organization
  • Fujita Kiyotaka
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University
  • Suganuma Toshihiko
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University

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タイトル別名
  • Starch Properties of Transgenic Sweetpotato Plants Modified by RNA Interference of the Starch Synthase II Gene

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説明

In this study, the physicochemical properties of starches from transgenic sweetpotato plants modified by RNA interference of the starch synthase II (SSII) gene were examined. The method of genetic manipulation developed during our previous study using White Star cultivar was applied to Konahomare cultivar, which has promising features of high yield and high starch content. SSII gene suppression resulted in the following consistent effects being observed in both cultivars: lowering of gelatinization temperature and gelatinization enthalpy, increase in B-type feature in X-ray diffractograms, decrease in the phosphate content of starch and alteration in chain-length distributions as determined by gel-permeation chromatography and high-performance anion-exchange chromatography. Furthermore, starches from all transgenic lines showed slower retrogradation and higher digestibility by glucoamylase compared to those from control starches. These traits of transgenic starches were similar to those from a starch of Quick Sweet cultivar, suggesting that mutation in the SSII gene could be one of the reasons for the unique property of starch in Quick Sweet. Thus, our RNA interference technique for sweetpotato was successfully applied to starch engineering in the promising cultivar of sweetpotato for starch material, Konahomare. The SSII gene inhibition significantly modified the basic architecture of starch and subsequently altered its functional properties.

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