Demonstration of Parotin in Thoracic Duct Lymph and Portal Vein Blood upon Intestinal Administration in the Rat

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  • MANITA HIDEAKI
    Department of Pharmacology, Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.
  • SUDO TADAMITSU
    Department of Pharmacology, Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.
  • ASANO HIDEICHI
    Department of Pharmacology, Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.

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The intestinal absorption of parotin was investigated in Wistar rats by immunochemical techniques and radio-isotope iodine 125 tracer method combined with gel filtration. After administration via intestine parotin levels with time in both thoracic duct lymph and portal vein blood were estimated.<BR>By immunochemical determination, percent of the dose absorbed in the lymph over 105 min upon administration of parotin with 5, 10, 20 and 50 mg doses per rat was 0.54, 0.30, 0.13 and 0.15 respectively. Portal vein blood level after administration of parotin (20mg/ml/rat) was only detectable in 90min serum sample, and was estimated to be 19.0μg/ml.<BR>The 125I-labelled parotin was injected via intestine and blood was collected in timed samples from the mesenteric vein. A radioactive peak was obtained at 60 min which corresponds to 0.96 % absorption of the initially administered radioactivity. When 125I was administered, its radioactivity reached maximum at 20 min. Gel filtration patterns of the serum sample collected at 60 min showed that 69.3% of the activity was concentrated in a large molecular fraction, while with the 20 min serum sample obtained after injection of 125I most of radioactivity was found in the second peak pattern, i. e. 125I fraction. From these results, it was presumed that 0.66 % of parotin was absorbed as large molecules through the intestine.<BR>The data obtained in the present experiment indicate that when administered via intestine parotin can be absorbed into thoracic duct lymph and portal vein blood stream with immunological reactivities and a large molecular size retained.

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