Dextran-Coated Charcoal Technique to Make the Hormone-Free Serum as a Diluent for Standard Curve of Radioimmunoassay

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  • OCHI YUKIO
    Second Department of Internal Medicine, Kyoto Prefectural University of Medicine
  • SHIOMI KATSUHIKO
    Second Department of Internal Medicine, Kyoto Prefectural University of Medicine
  • HACHIYA TAKASHI
    Second Department of Internal Medicine, Kyoto Prefectural University of Medicine
  • YOSHIMURA MANABU
    Second Department of Internal Medicine, Kyoto Prefectural University of Medicine
  • MIYAZAKI TADAYOSHI
    Second Department of Internal Medicine, Kyoto Prefectural University of Medicine

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Experiments were designed to make the hormone-free serum to serve for a standard curve of radioimmunoassay. The adsorption rate of hormone by dextran-coated charcoal (D. C. C.) was analysed using radioactive hormones such as 3, 5, 3' triiodothyronine (T3), thyroxine (T4), insulin, corticosterone, angiotensin I and II, ACTH, growth hormone (GH), TSH and HCG in pooled human serum. In general, the hormone of a small molecular weight was easily adsorbed into D. C. C., but the hormone of a large molecular weight needed a large amount of D. C.C. to remove hormone. Using the solution of high concentrated D. C. C.(20% charcoal and 2% dextran), T3, insulin, corticosterone and angiotensin I and II were adsorbed by more than 80% into a small amount of D. C. C. solution (0.1ml), T4, GH and ACTH were adsorbed by more than 80% into a relatively large amount of D. C. C. solution (1ml), and TSH and HCG were removed by 75% into a greater amount of D. C. C. solution (2ml) under 1ml of pooled serum. In this treatment of D. C. C. solution, serum albumin was removed slightly into charcoal.<BR>The standard curve of radioimmunoassay for TSH and GH was figured using the panhypopituitary patient serum (PPS) or PPS treated by D. C. C. The standard curve of TSH or GH using PPS did not remarkably differ from that of PPS treated by D. C. C. The hormone-free serum that was made by D. C. C. treatment is available for the standard serum for the double antibody radioimmunoassay of these hormones.

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