COMPARATIVE STUDIES ON THE PITUITARY HORMONES OF VERTEBRATES IV. EFFECTS OF CRUDE LH AND FSH ON THE OVULATION OF HYPOPHYSECTOMIZED NEWTS

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1. An injection of the fraction of swine, fowl, frog and salmon, which was precipitated at the step of 0.2 saturated ammonium sulphate solution, could induce ovulation of hypophysectomized newts. The minimum dose to the induce the ovulation was about 0.4mg in the swine and fowl, about 0.025mg in the frog and about 0.2mg in the salmon.<BR>2. The minimum dose of crude LH or crude FSH to induce ovulation of hypophysectomized newts was about 0.008mg in the swine, fowl and salmon, and about 0.004mg in the frog.<BR>3. A significant difference in effect on the ovulation of hypophysectomized newts is found between the crude LH and FSH of swine anterior pituitaries, when injected into the newts 14 days after hypophysectomy.<BR>4. The residue of 40% ethanol extraction of frog anterior pituitaries seems to contain some substances, which is synergistic with the crude LH and FSH of frog and salmon anterior pituitaries and antagonistic with those of swine and fowl ones in effect of the ovulation of hypophysectomized newts.

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