Partial separation of gonadotropin-inhibiting substance in human urine(Note)

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  • Partial Separation of Gonadotropin-Inhibiting Substance in Human Urine

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The chromatographic separation of gonadotropin-inhibiting substance in the extract obtained from adult male urine according to the method of Johnsen was attempted on a Sephadex G-100 column. The ovulation assay was employed to detect the inhibitor in the fractions using female mice, 19-20 days old, which received PMS and HCG to obtain a minimum constant ovulation. In the chromatography of the extract, the inhibitor mainly emerged in the early fractions or in the excluded volume, while HPG appeared in the later fractions which induced super-ovulation when injected with the standard dose of HCG. A slight inhibition on the ovulation was observed in the far later fractions.

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