Androphilic proteins in cytosols of human benign prostatic hypertrophy.
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- KODAMA TAKAOMI
- Department of Urology, School of Medicine, Chiba University
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- HONDA SEIKO
- Department of Urology, School of Medicine, Chiba University
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- SHIMAZAKI JUN
- Department of Urology, School of Medicine, Chiba University
書誌事項
- タイトル別名
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- Androphilic Proteins in Cytosols of Hum
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To examine the properties of androphilic proteins in human benign prostatic hypertrophy, the binding capacity and affinity of the proteins were determined after acetone-treatment, ammonium sulfate precipitation and chromatographies of DEAE and Sephadex G-200. Androphilic proteins in the extract of acetone-dried cytosol from the hypertrophic human prostate was precipitated at 30-50% saturation of ammonium sulfate. The binding of this fraction to dihydrotestosterone and testosterone was high affinity, but the binding to estradiol-17β was the one of non-specific. Androphilic proteins in the 30-50% fraction were eluted from DEAE cellulose column by buffer containing 0.05M KCl.<BR>On Sephadex G-200 chromatography of 30-50% fraction, the androphilic proteins were observed in three peaks; one was eluted in the void volume and other two were eluted at the sites of IgG and albumin. The amount and ratio of proteins eluted in the void volume and the site of IgG from Sephadex G-20Q column were variable in individual tissue samples. The chromatographic behavior of the 30-50% fraction in Sephadex G-200 was not changed significantly by introducing 0.4M KCl in the system. Polyacrylamide gel electrophoresis was applied for further separation of the proteins.
収録刊行物
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- Endocrinologia Japonica
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Endocrinologia Japonica 24 (6), 565-573, 1977
一般社団法人 日本内分泌学会
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詳細情報 詳細情報について
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- CRID
- 1390282681276571136
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- NII論文ID
- 130000954875
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- NII書誌ID
- AA00635052
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- ISSN
- 21856370
- 00137219
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- NDL書誌ID
- 1916958
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- PubMed
- 75101
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可