Electrofocusing of Partially Purified Bovine Luteinizing Hormone

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  • Electrofocusing of partially purified bovine luteinizing hormone(Note)

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Activity of luteinizing hormone (LH) was separated into two major components and an additional minor component by isoelectrofocusing of a partially purified bovine adenohypophysial preparation obtained by the gel filtration with Sephadex G-100. The components posessed the isoelectric point at pH 8.8, 8.3, and about 4.0, respectively. This result shows that bovine LH consists of two or three kinds of molecules with different isoelectric points.

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