THE EFFECTS OF PURIFIED SALIVA-PAROTIN-A ON THE DEVELOPMENT OF 9-DAY CHICK EMBRYO FEMORA GROWING IN TISSUE CULTURE

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  • ITO YOSOJI
    Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
  • ENDO HIROYOSHI
    Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
  • ENOMOTO HIROSHI
    Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo

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タイトル別名
  • STUDIES ON THE PHYSIOLOGICAL CHEMISTRY OF THE SALIVARY GLANDS LIV

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説明

The effects of purified saliva-patorin-A on the development of 9-day chick embryo femora growing in vitro were studied. Pair-mate bones were cultivated for 6 days by the roller-tube culture method without plasma. One of each pair was grown in the medium consisting of 1 part of chick embryo extract, 2 parts of horse serum and 7 parts of saline solution, and another in the plus-purified salivaparotin-A medium.<BR>The longitudinal growth of the bones was determined from measurement of the over-all length recorded every day throughout the cultivation period. The dry weight of the bones was also obtained. The degree of calcification of the bones was estimated from determination of the phosphorus deposited as bone salt. Finally the formation of cartilage matrix was evaluated from estimation of the amount of hydroxyproline in the acid hydrolysate of the bones.<BR>These experiments resulted in the following;(a) on the longitudinal growth of the bones, purified saliva-parotin-A exhibited a slightly stimulative tendency at concentrations of 0.05 and 0.1μg/ml, (b) on the dry weight and the amount of hydroxyproline, no clear directed change was found within the concentrations ranging from 0.01 to 10μg/ml and a marked decrease was observed at concentration of 50μg/ml, (c) on the amount of phosphorus, however, evident increase was found over a wide range of concentration from 0.01 to 1.0μg/ml, while at concentration of 50μg/ml a marked decrease was seen.<BR>From these findings it was suggested that purified saliva-parotin-A has a direct stimulative effect selectively upon calcification of the bone under certain optimal condition, though the overdoses of it retard the over-all growth of the bone.

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