Relationship of Mitochondrial Enzyme Activity to Chilling Injury of Cucumber Fruit

DOI JASI Web Site Web Site 1 Citations 13 References

Bibliographic Information

Other Title
  • キュウリ果実の低温障害とミトコンドリアの酵素活性との関係
  • キュウリ カジツ ノ テイオン ショウガイ ト ミトコンドリア ノ コウソ カ

Search this article

Abstract

For clarifying the process of chilling injury in cucumber fruit, organelles were isolated by means of sucrose density gradient centrifugation, and the profiles of distribution of activities of marker enzymes, especially in mitochondria, on the sucrose density gradient were analyzed. The fruit packed with perforated polyethylene bag was stored at 1°C and 15°C. Pitting on the surface of the fruit, a visible symptom of chilling injury, was observed after 5 days of storage at 1°C, whereas in the fruit stored at 15°C, sound appearances were kept for more than 7 days. The fruit stored at 1°C for 3 days was healthy, and even after transferred to 15°C no pitting injury developed. The rate of K+ ion leakage into desalted water from the section prepared from the fruit stored at 1°C increased linearly as storage time increased, indicating that an increase of membrane permeability may occur preceding the development of pitting.<br>The activities of cytochrome c oxidase located in inner membrane of mitochondria and malate dehydrogenase located in matrix decreased before the development of pitting. This suggests that the lowering of the function of mitochondria took place at low temperature before the occurrence of visible symptom of injury. When the fruit was transferred to 15°C at early stage of low temperature storage (3 days at 1°C), a recovery of the enzyme activities was recognized.<br>The activity of NADH cytochrome c reductase located in outer membrane of mitochondria increased soon during the storage at 1°C, and broadening of distribution of this enzyme on sucrose density gradient was observed with the fruit which exhibited pitting injury, while the activity of the enzyme taken from the fruit stored at 15°C decreased during the storage.

Journal

Citations (1)*help

See more

References(13)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top