Studies on resistance of black spot disease fungus of Japanese pear (<i>Alternaria kikuchiana</i> TANAKA). VII

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  • 日本ナシの黒斑病菌 (<i>Alternaria kikuchiana</i> TANAKA) に対する抵抗性に関する研究 (第7報)
  • 日本ナシの黒斑病菌(Alternaria Kikuchiana TANAKA)に対する抵抗性に関する研究-7-自然病斑からhost-specific toxinの単離とそのtoxinの毒性軽減物質について
  • ニホン ナシ ノ クロ ハン ビョウキン Alternaria Kikuchiana TANAKA ニ タイスル テイコウセイ ニ カンスル ケンキュウ 7 シゼン ビョウハン カラ host-specific toxin ノ タンリ ト ソノ toxin ノ ドクセイ ケイゲン ブッシツ ニ ツイテ
  • Studies on resistance of black spot disease fungus of Japanese pear (Alternaria kikuchiana TANAKA). VII
  • The isolation of host-specific toxin from the natural lesions, and the chemicals which decrease in toxicity of that toxin

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The isolation of host-specific toxin from natural lesions attacked the young leaves of Nijisseiki, susceptible variety, by black spot disease fungus of Japanese pear (Alternaria kikuchiana TANAKA) and decrease in toxicity of that toxin with various chemicals was described.<br>The isolation of K-III fraction (host-specific toxin) from culture filtrate and mycelium of causal fungus was previously reported. The toxin isolated from natural lesions was identified that fraction with thin layer chromatography and plant test, so that the fraction was regarded as vivotoxin.<br>Toxicity of that fraction was decreased with tannic acid, pyrogallol, hydroxyhydroquinone, pcresol and dimethyl sulfoxide. Effect of tannic acid on toxicity of that fraction was excelled. Toxicity of that fraction (200ppm) was completely inhibited with tannic acid of 10, 000ppm and moderately inhibited with the same substance 1, 000 and 100ppm. The consequent decrease in necrosis on the young leaves was depened upon the relative concentration of both that fraction and tannic acid.

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