リンゴ台木'マルバカイドウ長野No.1'におけるAgrobacterium rhizogenesにより形成された毛状根からの植物体再分化

DOI NDLデジタルコレクション 日本農学文献記事索引 Web Site Web Site 被引用文献1件 参考文献36件

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タイトル別名
  • Plant Regeneration from Hairy Roots of Apple Rootstock, Malus prunifolia Borkh. var. ringo Asami, Strain Nagano No.1, Transformed by Agrobacterium rhizogenes

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Stems of Mains prunifolia Borkh. var. ringo Asami strain Nagano No.l were infected with Agrobacterium rhizogenes MAFF-02-10265, MAFF-02-10266, MAFF-03-01724 and MAFF-03-01725 by using a needle. The frequency of hairy root formation was 2.0% with both MAFF-02-10265 and MAFF-02-10266. Transgenic plants were obtained from hairy roots that were induced by A. rhizogenes MAFF-02-10265. Adventitious shoots were formed from the hairy roots on Murashige and Skoog medium, supplemented with 5.0 mg·liter-1 6-benzyl-aminopurine (BAP), 0.1 mg·liter-1 indole-3-butyric acid (IBA) and 0.1 mg·liter-1 gibberellic acid (GA3). These shoots developed abundant roots in the culture medium. The transgenic nature of the regenerated plants was confirmed by opine and PCR analysis. A transgenic plant, acclimatized in the greenhouse, showed prolific branching, miniaturized leaves, and shortened internodes.

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  • 園芸学会雑誌

    園芸学会雑誌 73 (6), 505-510, 2004

    一般社団法人　園芸学会

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