Rhizome Formation in <i>Cymbidium goeringii</i> Reichenbach fil. and <i>Cymbidium kanran</i> Makino in Shoot-Tip Culture

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  • 茎頂培養によるシュンラン及びカンランのライゾーム形成
  • 茎頂培養によるシュンラン及びカンランのライゾーム形成〔英文〕
  • ケイチョウ バイヨウ ニ ヨル シュンラン オヨビ カンラン ノ ライゾーム
  • Rhizome Formation in Cymbidium goeringii Reichenbach fil. and Cymbidium kanran Makino in Shoot-Tip Culture

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Abstract

Cymbidium goeringii Reichenbach fil. and Cymbidium kanran Makino are native Cymbidia of Japan and are important Oriental terrestrial orchids. When their shoot-tips are cultured, rhizomes are formed. Factors affecting rhizome formation were discussed in this paper.<br>1. Culturing of shoot-tips of Cymbidium goeringii at intervals of 2 months showed that rhizomes could be formed at any time throughout the year. However, rhizome formation depended on the starting time of cultures and the culture media. Maximum rhizome formation occurred in April when cultured in either MS or RM media both containing 1ppm NAA and 0.1ppm kinetin, and in June by culturing with modified Cymbidium-culture medium (Hyponex medium) devoid of plant growth regulators.<br>2. Culturing the shoot-tips of Cymbidium goeringii in darkness at 25°C hastened rhizome formation, and increased rhizome formation to almost twice the level obtained under conventional culture conditions with artificial illumination for 16 hours.<br>3. RM medium containing 1/5 concentration of inorganic components and 10% coconut water was found to be suitable for shoot-tip culture of Cymbidium kanran.<br>4. When shoot-tips of Cymbidium kanran were cultured in agitated liquid medium, all explants died.<br>5. The use of plant growth regulators was found to have no effect in cultures of Cymbidium goeringii and Cymbidium kanran.

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