Cultivar Identification of Evergreen Azalea with RAPD Method.

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  • Kobayashi Nobuo
    Institute of Agriculture and Forestry, University of Tsukuba
  • Takeuchi Rieko
    Institute of Agriculture and Forestry, University of Tsukuba Agriculture and Forestry Division of Miyazaki Prefecture
  • Handa Takashi
    Institute of Agriculture and Forestry, University of Tsukuba
  • Takayanagi Kenji
    Institute of Agriculture and Forestry, University of Tsukuba

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Other Title
  • RAPD法による常緑性ツツジの品種同定
  • RAPD法による常緑性ツツジの品種同定〔英文〕
  • RAPDホウ ニヨル ジョウリョクセイ ツツジ ノ ヒンシュ ドウテイ エイブ

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Abstract

To use RAPD method for the identification of evergreen azalea cultivars, the optimum method for DNA extraction in Rhododendron spp. was investigated. Of almost all Rhododendron species native to Japan and some cultivar groups, neither SDS nor CTAB method was sufficient to isolate clean DNA from leaves. But, by using the modified CTAB method after Greenwood et al. (1989), we obtained a good yield of high quality DNA from the materials used in this experiment.<BR>Using the optimum extraction method mentioned above and RAPD analysis, differences among azalea cultivars could be distinguished. Sixteen out of 100 primers tested produced obvious, definitive polymorphic DNA bands. Cultivars in the Edo-Kirishima group (9 cultivars) or Kurume azalea group (14 cultivars) were clearly recognized from one another by using primer OPK-19 or OPK-20. In the Satsuki group, these two primers could be used to discern 'Kouzan' from its bud sport cultivars.<BR>The results of this study strongly suggest that RAPD method is a very useful tool for cultivar identification in evergreen azalea.

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