紫外線を照射したブドウ果皮におけるリスベラトロール合成の誘導

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  • Induction of Resveratrol Biosynthesis in Skins of Three Grape Cultivars by Ultraviolet Irradiation

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Resveratrol production and expression of the genes related to resveratrol biosynthesis were investigated in the skins of three Vitis vinifera cultivars Chardonnay, Koshu and an American hybrid grape, Muscat Bailey A (Bailey × Muscat Hamburg). Resveratrol concentration in the skins of all the grapes increased significantly when exposed to ultraviolet (UV-C, 254 nm) irradiation. The UV-induced resveratrol concentration in the grape skins was lower after veraison (onset of ripening) than before it. The maximum concentration of the UV-induced resveratrol in 'Muscat Bailey A' was higher than those in the other two cultivars. The relative mRNA expression levels of stilbene synthase (STS), phenylalanine ammonia-lyase (PAL) and chalcone synthase (CMS) genes in grape skins 8 hr after UV irradiation were determined by quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results revealed that STS- and PAL-mRNA expressions were significantly increased by UV irradiation. STS-mRNA expressions in 'Muscat Bailey A' were higher than those in 'Chardonnay' throughout berry development. The UV-induced CMS-mRNA expression in the grape skins decreased before veraison and subsequently increased.

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  • 園芸学会雑誌

    園芸学会雑誌 73 (3), 193-199, 2004

    一般社団法人 園芸学会

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