Molecular Cloning of Vacuolar H+-ATPase A Subunit Paralogs and their Expression in Pear Fruit
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- Amemiya Tsuyoshi
- Graduate School of Bioagricultural Sciences, Nagoya University
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- Suzuki Yasuo
- Faculty of Agriculture, Kobe University
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- Yamaki Shohei
- Graduate School of Bioagricultural Sciences, Nagoya University
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- Shiratake Katsuhiro
- Graduate School of Bioagricultural Sciences, Nagoya University
Bibliographic Information
- Other Title
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- セイヨウナシ果実の液胞型H<sup>+</sup>-ATPase A subunit cDNAパラログのクローニングとその発現解析
- Molecular cloning of vacuolar H〔+〕-ATPase A subunit paralogs and their expression in pear fruit
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Abstract
We cloned two cDNAs (PcVHA-A1, PcVHA-A2) encoding V-ATPase A subunit (catalytic subunit) in pear (Pyrus communis L. var. sativa DC, ‘La France’). They encode proteins with a calculated molecular weight of approximately 69 kDa, which consist of 623 amino acids. The expression of PcVHA-A1 mRNA was extremely high in mature fruit and young leaves, but low in flower, young fruit, mature leaves, and root, it increased with the maturation of pear fruit. On the other hand, the expression of PcVHA-A2 mRNA was relatively high at the early and late developmental stages of the pear fruit. A certain level of PcVHA-A2 expression was also observed in flowers and leaves.
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 74 (3), 258-260, 2005
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Keywords
Details 詳細情報について
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- CRID
- 1390282681279058944
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- NII Article ID
- 110001815990
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- NII Book ID
- AN00025257
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- ISSN
- 1880358X
- 00137626
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- NDL BIB ID
- 7363145
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed