Pollen-Stigma Interaction Which Might Be Critical to the Gametophytic Incompatibility of Japanese Pear

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  • ニホンナシの不和合性における柱頭の関与
  • ニホンナシの不和合性における柱頭の関与〔英文〕
  • ニホンナシ ノ フワゴウセイ ニ オケル チュウトウ ノ カンヨ エイブン

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Abstract

A few fundamental experiments were carried out to increase information about the incompatibility of Japanese pear (Pyrus serotina R.). The results obtained are summarized as follows.<br>1. Flowers of ′Kosui′ at anthesis were self- or cross-pollinated after emasculation, and pollen germination on stigmas at 6h after pollination was observed. Pollen grains in cross-pollination germinated well and their pollen tubes elongated into stigmas by that time. But in self-pollination, the number of pollen grains observed on the stigma surface was very small and few pollen grains stretched their tubes into stigmas. Tube growth of cross-pollen was smooth in styles and some of the pollen tubes penetrated into ovaries 72h after pollination. On the contrary, tube growth of self-pollen was very poor and most of the tube tips stayed around 2mm below the stigma surface at 72h after pollination.<br>2. Flowers of ′Kosui′ and ′Chojuro′ at anthesis were emasculated, self- or cross-pollinated, fixed at intervals, stored in 70% Ethanol and processed for fluorescent microscopy through a routine procedure, where the number of pollen grains left adhered to the stigma surface was counted. On ′Kosui′, cross-pollination greatly surpassed self-pollination at 3h after pollination. But the difference decreased as time went on and there existed no difference between self- and cross-pollination at 12h after pollination. On ′Chojuro′, cross-pollination was superior to self-pollination from 6 to 12h after pollination.<br>3. Pistils of ′Kosui′ that were sampled soon after pollination were analyzed for proteins by IEF electrophoresis in combination with a silver nitrate staining procedure. Clear difference was observed between self- and cross-pollination as to the band pattern of buffer-soluble proteins of ovaries as soon as at 3h after pollination.<br>From these results, it was suggested that the stigma might play some significant role in the function of incompatibility of Japanese pear.

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