Identification of chloroplast DNA variations by PCR-RFLP analysis in Dendranthema

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  • キク属植物におけるPCR-RFLP法による葉緑体遺伝子変異の同定

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Abstract

Variations in chloroplast DNA of Dendranthema species were studied by PCR-RFLP analysis to seek the maternal origin of the cultivated chrysanthemum, D. grandlflorum. Ten genes, atpH, matK, petA, petB, psaA, rbcL, rpoE, rpoC, trnK and 76S in the chloroplast DNA of 12 Japanese wild species and 1 cultivar were amplified by PCR. The amplified DNAs that were digested with each of 32 restriction endonucleases revealed 13 site changes among the 13 species in the following 9 gene plus restriction endonuclease combinations: petA-Avall, petA-Haelll, petA-MboII, petA-NdeII, rpoC-EcoRV, trnK-Dral, trnK-HinfI, trnK MboII and trnK-ScrFI. No Japanese wild species showed the same PCR-RFLP pattern of chloroplast DNA as D. grandiflorum.

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