Seasonal Changes in the Activities of Vacuolar H+-pumps and their Gene Expression in the Developing Japanese Pear Fruit.

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  • ニホンナシ果実の生長過程での液胞膜プロトンポンプの活性の変動と遺伝子発現

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Abstract

Vacuolar H+-ATPase (V-ATPase) and vacuolar H+-inorganic pyrophosphatase (V-PPase) generate an electrochemical gradient across the vacuolar membrane, which is indispensable for accumulating sugars into the vacuole. We investigated the enzymatic and transcriptional changes of V-ATPase and V-PPase in Japanese pear fruit to establish their roles in fruit development. As fruit developed, the V-ATPase and V-PPase activities increased on the total protein basis. The V-PPase activity was higher than that of V-ATPase throughout the developmental period. Immunoblot analyses indicated that the changes in the polypeptides for the catalytic subunit of V-ATPase and V-PPase generally paralleled the enzymatic activities. Furthermore, seasonal changes in the mRNA levels generally corresponded with those of polypeptides except at the cell division stage. Our findings suggest that V-PPase, more than V-ATPase, plays an important role throughout the development of Japanese pear fruit and that V-ATPase and V-PPase are regulated at the transcriptional level during the cell elongation stage.

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