Fluorescent Banding Pattern of Peach (Prunus persica (L.) Batsch) Chromosomes.

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  • モモ染色体の蛍光染色

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Abstract

The chromomycin A3 (CMA) and 4'-6-diamidino-2-phenylindole (DAPI) chromosome banding patterns of peach (Prunus persica (L.) Batsch) (2n=16) were determined on root tips of open-pollinated seedlings of three cultivars : 'Ohatsumomo', 'Akatsuki', and 'Momo daigi tsukuba 4 gou' ('Tsukuba No.4'). Root tips were pretreated in 2mM 8-hydroxyquinoline at 10°C for 4hr ; the chromosome samples were prepared by the enzymatic maceration and air-drying method. CMA positive (+) and DAPI negative (-) bands were observed in the satellite position of two chromosomes, terminal position of two chromosomes, and proximal position of two chromosomes. Ten chromosomes did not have any CMA+/DAPI-band. The number and positions of CMA+/DAPI-bands were stable, and there was no difference among the seedlings from the three cultivars. No CMA-/DAPI+band was observed.

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