Gene Expression of NAD+-Dependent Sorbitol Dehydrogenase and NADP+-Dependent Sorbitol-6-Phosphate Dehydrogenase During Development of Loquat(Eriobotrya japonica Lindl.) Fruit.
Bibliographic Information
- Other Title
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- ビワ果実の生長・成熟に伴う NAD<SUP>+</SUP> 依存性ソルビトール脱水素酵素と NADP<SUP>+</SUP> 依存性ソルビトール-6-リン酸脱水素酵素の遺伝子発現
- Gene expression of NAD〔+〕-dependent sorbitol dehydrogenase and NADP〔+〕-dependent sorbitol-6-phosphate dehydrogenase during development of loquat (Eriobotrya japonica Lindl.) fruit
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Abstract
The aim of this study is to clarify the roles of NAD+-dependent sorbitol dehydrogenase (NAD-SDH) and NADP+-dependent sorbitol-6-phosphate dehydrogenase (S6PDH) in fruit development, and the regulatory mechanism (s) underlying their expression during development using loquat as the study material. The cDNA of NAD-SDH, cloned from loquat fruit, consisted of 1, 572 bp and contained an open reading frame of 1, 023 bp capable of encoding a protein of 371 amino acids. The deduced amino acid sequence has 97.8% identity to that of an apple fruit. The activity of NAD-SDH is a function of fruit development, i.e. the increase in protein of this enzyme synchronized consistently with that of activity showing no posttranslational modification throughout the developmental stages. Furthermore, NAD-SDH activity correlated with the mRNA levels, indicating that the key regulatory step of the activity is at the transcriptional level. The increase in NAD-SDH with fruit development plays a dominantly important role during fruit maturation and sugar accumulation. The trend of S6PDH activity during fruit development paralleled that of NAD-SDH activity. It correlated with protein and mRNA levels revealing that the regulation of the activity is mainly at the transcriptional level like NAD-SDH. However, the role of S6PDH in fruit is not yet clear.
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 69 (3), 231-236, 2000
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681279557376
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- NII Article ID
- 110001816385
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- NII Book ID
- AN00025257
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- COI
- 1:CAS:528:DC%2BD3cXjsValtL4%3D
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- ISSN
- 1880358X
- 00137626
- http://id.crossref.org/issn/00137626
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- NDL BIB ID
- 5396546
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- Abstract License Flag
- Disallowed