Plant regeneration from mesophyll protoplasts of tomato cv. Ponderosa

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  • トマト葉肉プロトプラストからの植物体再生
  • トマト葉肉プロトプラストからの植物体再生〔英文〕
  • トマト ヨウニク プロトプラスト カラ ノ ショクブツタイ サイセイ エイブン

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Axenic seedlings of tomato cv. Ponderosa were used as material for protoplast culture. Mesophyll protoplasts were treated in an enzyme solution containing 0.5% Cellulase“Onozuka”RS and 0.1% Pectolyase Y-23 for 3.5hr. at 27°C at 40spm. Protoplasts were incubated in a liquid medium containing one-fourth strength mineral salts of MS medium(8), organic components of 8p medium(5), 1.0mg/l NAA and 0.5mg/l BA at 29°C in the dark. After the first cell division, the culture was transferred to a light intensity of 300lux at 25°C. By adding mannitol-free medium stepwise after the first or second cell division, protoplasts were grown to small calluses 0.5-1.0mm in diameter) by the 30th day. The calluses, transplanted onto agar medium containing MS basal medium, 1.0mg/l Zeatin and 0.02mg/l GA, differentiated adventitious buds within 18 days. Regenerated shoots formed roots on MS agar medium with 0.2mg/l GA, and developed into fertile plants.

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