Isolation and Analysis of mRNAs Relating to Citrus Rind Injury under Various Storage Conditions.

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  • 様々な貯蔵条件におけるカンキツ果皮障害に関連するmRNAの単離とその発現

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Abstract

A differential display technique that uses an arbitrary sequence primer, labeled with digoxigenin, was applied to detect differentially expressed mRNAs relating to citrus rind injury under various storage conditions. mRNAs were isolated and identified, some of which share a high similarity with those detected in plant organs by a virus infection, elicitor treatments, or water-stress, whereas others share homology with auxin-inducible genes. Expressions of three genes were analyzed by a reverse transcriptase-polymerase chain reaction (RT-PCR) in the rind of 'Kiyomi' tangor (Citrus unshiu Marc. × C. sinensis Osb.). The three genes were induced early under conditions that favored severe rind injury after a lengthy storage. Differential expressions of those genes should be useful to identify unfavorable storage conditions for citrus fruit.

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