Varietal Variations in Plant Regenerative Potential from Protoplasts in Spinach (Spinacia oleracea L.).

DOI NDL Digital Collections JASI Web Site Web Site 2 Citations 13 References

Bibliographic Information

Other Title
  • ホウレンソウプロトプラストからの植物体再生における品種間差異
  • Varietal variations in plant regenerati

Search this article

Abstract

The regenerative potential of seven Spinacia oleracea L. varieties in protoplast culture was compared. Although protoplasts were isolated from all cultivars, the yield of protoplast, plating efficiency (PE), and colony formation ratio were remarkably different among them. The highest PE and colony formation ratio were obtained from protoplasts of 'Ujo', followed by 'Jiromaru', 'Hoyo', and 'Minsterland', which exceeded those of 'Sapporo-oba', 'Viroflay', and 'Viking'. Shoots were regenerated by 'Ujo', 'Jiromaru', 'Hoyo', and 'Minsterland'. 'Hoyo' and 'Jiromaru' had the highest frequency ; no shoot developed from the calli of 'Sapporo-oba', 'Viroflay', and 'Viking'. The highest frequency of shoot regeneration was obtained in a media containing 1.0 or 10.0mg·liter-1 IBA, but at 10.0mg·liter-1 IBA or IAA, the shootlets were often vitrified and fasciated.

Journal

Citations (2)*help

See more

References(13)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top