HISTOCHEMICAL STUDIES ON EXPERIMENTAL TYPHOID BY MEANS OF FLUORESCEIN‐LABELED ANTIBODY
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- TANAKA NOBUO
- Institute of Applied Microbiology and Department of Pathology, Faculty of Medicine, University of Tokyo
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- NISHIMURA TOSHIO
- Institute of Applied Microbiology and Department of Pathology, Faculty of Medicine, University of Tokyo
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- YOSHIYUKI TOSHIYUKI
- Institute of Applied Microbiology and Department of Pathology, Faculty of Medicine, University of Tokyo
書誌事項
- タイトル別名
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- HISTOCHEMICAL STUDIES ON EXPERIMENTAL TYPHOID BY MEANS OF FLUORESCEIN-LABELED ANTIBODY
- I. CELLULAR LOCALIZATION OF THE ANTIGENS OF SALMONELLA ENIERITIDIS AFTER INJECTION OF THE KILLED VACCINES
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説明
The cellular localization of the antigens of Salmonella enteritidis in the mouse tissues after intravenous and intraperitoneal injections of the killed organisms was investigated, using the double-layer method of fluorescein-labeled antibody technique (Coons). The heat-killed organisms and the chrome-vaccine were comparatively studied. In the liver, the antigens of Salmonella enteritidis were observed to fill the cytoplasms of the Kupffer cells, and those of the leukocytes (monocytes and granulocytes) infiltrated around the sinusoids and other vessels. When the granulomas were formed, the antigens were demonstrated in their cells in various amounts; some contained a large amount of the antigens and the others small. The epitheloid and other cells, which constituted the granulomas, contained the antigens in their cytoplasms. In the spleen, the antigens were localized in the cytoplasms of the large round mononuclear cells (chiefly reticulum cells) of the red pulp. Using heatkilled vaccine, the antigens accumulated in a few layers of cells lining the lymphoid follicles. However, after administering chrome-vaccine, it was found in the cells of the red pulp. The other differences in the distribution of the antigens between the heat-killed vaccine and the chrome-vaccine were described. The localization of antigenic particles of the killed organisms was markedly different from that of the endotoxin (the soluble antigen). Biological significance of the distribution of the antigens was discussed, particularly the role of antigen and antibody in the formation of the granuloma.
収録刊行物
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- Ｊａｐａｎｅｓｅ Ｊｏｕｒｎａｌ ｏｆ Ｍｉｃｒｏｂｉｏｌｏｇｙ
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Ｊａｐａｎｅｓｅ Ｊｏｕｒｎａｌ ｏｆ Ｍｉｃｒｏｂｉｏｌｏｇｙ 3 (3), 267-276, 1959
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