Enhanced Growth and Plaquing of Rabies Virus in Static Chick Embryo Cell Culture

DOI Web Site Web Site Web Site PubMed 被引用文献1件 参考文献31件

書誌事項

タイトル別名
  • Enhanced growth and plaquing of rabies

この論文をさがす

説明

The 7-day egg passage line of HEP Flury strain of rabies virus was inoculated to primary chick em-bryo (CE) cells prepared in different ways to compare efficiencies of viral growth and plaquing. Special care to minimize cellular damage due to trypsin at the step of monodispersion and sowing a comparatively large number of cells for monolayer preparation were required for rabies plaquing, whereas such cares were not necessary for plaquing of vesicular stomatitis virus. Plaque number and size were increased by incorporation of a high concentration of thymidine into cell growth medium. Various other means to produce a static state of CE cells were tested, and a maximal plaquing effi-ciency was obtained when dishes receiving a massive number of dispersed cells in MEM plus 1% calf serum were incubated at 37 C for 1 day without any buffering for monolayer preparation and post-infection incubation was done at 32 C in a CO2-incubator. Bottle cultures of CE cells prepared in a similar manner, when infected with HEP Flury virus, yielded a markedly higher titer of virus than CE cells prepared by our previous standard method.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (31)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ