PIGMENT PRODUCTION BY <i>PSEUDOMONAS AERUGINOSA</i> ON GLUTAMIC ACID MEDIUM
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- OSAWA SHINOBU
- Department of Microbiology, Kansai Medical School Liberal Arts Department, Chemistry, Kansai Medical School
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- YABUUCHI EIKO
- Department of Microbiology, Kansai Medical School Liberal Arts Department, Chemistry, Kansai Medical School
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- NARANO YOSHIË
- Department of Microbiology, Kansai Medical School Liberal Arts Department, Chemistry, Kansai Medical School
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- NAKATA MINAKO
- Department of Microbiology, Kansai Medical School Liberal Arts Department, Chemistry, Kansai Medical School
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- YUKAWA YOKO
- Department of Microbiology, Kansai Medical School Liberal Arts Department, Chemistry, Kansai Medical School
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- TAKASHINA KIYOKO
- Department of Microbiology, Kansai Medical School Liberal Arts Department, Chemistry, Kansai Medical School
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- TANABE TOYOKO
- Department of Microbiology, Kansai Medical School Liberal Arts Department, Chemistry, Kansai Medical School
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- TOMONO HAJIME
- Department of Microbiology, Kansai Medical School Liberal Arts Department, Chemistry, Kansai Medical School
書誌事項
- タイトル別名
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- PIGMENT PRODUCTION BY PSEUDOMONAS AERUGINOSA ON GLUTAMIC ACID MEDIUM
- II. STUDIES ON FLUORESCENCE I
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説明
1) The fluorescent substance produced by Pseudomonas aeruginosa was studied using strains which possess a charcteristic pigment-producing ability, strains No. 134, 145 and 117.<br>2) The Fluorescence I production of strain No. 134 is rapid and pronounced. <br>The effect of pH on the yellow-green portion of the Fluorescence I of this strain was studied and the following results were obserbed:<br>i) A reversible change in the tone of fluorescence takes place between pH 5.0 and pH 10.0. The color changes from blue to yellow-green at about pH 7.5 with a clear demarcation.<br>ii) The absorption spectrum of the substance shows a peak at 400mμ, and a characteristic wave length shows a peak at 460mμ. A change is noted in the absorption spectrum and characteristic wave length at about pH 5.0-4.0 when the tone of the fluorescence changes from colorless to orange.<br>3) The Fluorescence I fraction obtained by gel filtration from the culture filtrate of the three strains grown on the glutamic acid medium was examined paper-electrophoretically and the following results were obtained:<br>i) Four fluorescent spots appear on the filter paper. The spots were arbitrarily called α, β, γ and δ from the negative pole side. Strain 117 shows α and β and strains 134 and 145 shows β, γ and δ<br>ii) These spots appear in order from the negative pole side with time of cultivation regardless of the strain.
収録刊行物
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- Ｊａｐａｎｅｓｅ Ｊｏｕｒｎａｌ ｏｆ Ｍｉｃｒｏｂｉｏｌｏｇｙ
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Ｊａｐａｎｅｓｅ Ｊｏｕｒｎａｌ ｏｆ Ｍｉｃｒｏｂｉｏｌｏｇｙ 8 (4), 115-123, 1964
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