Epidemiological Survey of <i>Streptococcus mutans</i> among Japanese Children

  • HAMADA Shigeyuki
    Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School Department of Microbiology, Northwestern University Medical and Dental School
  • MASUDA Norio
    Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School
  • OOSHIMA Takashi
    Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School
  • SOBUE Shizuo
    Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School
  • KOTANI Shozo
    Department of Oral Microbiology and Department of Operative Dentistry, Pedodontic Clinic, Osaka University Dental School

書誌事項

タイトル別名
  • Epidemiological Survey of Streptococcus mutans among Japanese Children
  • Identification and Serological Typing of the Isolated Strains

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説明

An epidemiological investigation was carried out to identify and determine the serotypes of Streptococcus mutans from carious lesions of young Japanese children. For this purpose, a direct fluo-rescent antibody technique was mainly used. Fluorescein isothiocyanate-conjugated antibodies were prepared for the five known serotypes of S. mutans. Cross reactions and nonspecific reactions were eliminated by adsorption, counterstaining, or DEAE-cellulose column chromatography. Agar-gel immunodiffusion was used to distinguish between serotypes a and d. The epidemiological survey suggested that serotype c strains were most prevalent in dental plaques of Japanese children. The d and e serotypes were rare and serotypes a and b were not detected. It was also noted that more than one serotype of S. mutans could be found in the same locus of a carious lesion and that there might be no relationship between the degree of caries and the causative serotype(s) of S. mutans.

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