Studies on Delayed Hypersensitivity of Antigens Isolated from <i>Mycoplasma pneumoniae</i> Cells

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  • SUZUKI Masuko
    Department of Microbiology, Tohoku College of Pharmacy Department of The First Internal Medicine, Tohoku University School of Medicine Department of Microbiology, Tohoku University School of Dentistry
  • HAYASHI Yoshiro
    Department of Microbiology, Tohoku College of Pharmacy Department of The First Internal Medicine, Tohoku University School of Medicine Department of Microbiology, Tohoku University School of Dentistry
  • ARAI Sumio
    Department of Microbiology, Tohoku College of Pharmacy Department of The First Internal Medicine, Tohoku University School of Medicine Department of Microbiology, Tohoku University School of Dentistry
  • KUMAGAI Katsuo
    Department of Microbiology, Tohoku College of Pharmacy Department of The First Internal Medicine, Tohoku University School of Medicine Department of Microbiology, Tohoku University School of Dentistry

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タイトル別名
  • Studies on Delayed Hypersensitivity of Antigens Isolated from Mycoplasma pneumoniae Cells
  • Studies on delayed hypersensitivity of

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Cells of Mycoplasma pneumoniae Mac strain were fractionated into acetone-soluble and insoluble fractions. Acetone-insoluble fractions were digested with pronase and further purified by chromatography on Sephadex G-75, yielding three water-soluble fractions which were free from lipid and consisted mainly of polysaccharide-protein complex. All these water-soluble fractions possessed eliciting antigenicity to delayed hypersensitivity for M. pneumoniae as measured by skin reactions and macrophage migration inhibition tests, but not to complement-fixing antibodies. In contrast, the acetone-soluble fraction was reactive with the complement-fixing antibodies but not for the delayed hypersensitivity.

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