Magnesium-Stimulated Resynthesis of Osmoresistant Layer by <i>Candida albicans</i> Spheroplasts

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  • OTA Fusao
    Department of Bacleriology. Tokushima Universily School of Medicine.

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  • Magnesium-stimulated resynthesis of osmoresistant layer by Candida albicans spheroplasts
  • Magnesium‐Stimulated Resynthesis of Osmoresistant Layer by <i>Candida albicans</i> Spheroplasts

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Abstract

Spheroplasts of Candida albicans could be obtained by treating cells with a snail enzyme in the presence of 0.75M MgSO4 as a stabilizer and cells could subsequently be regenerated by the thin-layer-agar plating method. The initial regeneration process was affected by stabilizers such as MgSO8 MgCl2, MnSO4, CaCl2, KCl, NaCl and sucrose. It was found that 0.68M MgSO4 was the best stabilizer. Good stabilization was also achieved in any medium with 0.5-0.75M sucrose, although stabilizing agents were in general most effecive at concentrations giving about 22 atmospheres of osmolarity. This osmolarity was lower than that used to prepare spheroplasts. Using this thin-layer plating method at least three factors were essential for resynthesis of a wall layer: (1) a carbon source, (2) a stabilizer and (3) several minerals. Nitrogen in the form of an amino acid stimulated formation of the wall layer. Relationship between these factors and osmolarity of the medium during regeneration of the wall layer is discussed.

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