Use of a Diffusion Barrier Technique in Bioautography
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- NAKAMURA Hiroshi
- Faculty of Pharmaceutical Sciences, University of Tokyo
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- SAMEJIMA Keijiro
- Faculty of Pharmaceutical Sciences, University of Tokyo
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- TAMURA Zenzo
- Faculty of Pharmaceutical Sciences, University of Tokyo
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Abstract
A diffusion barrier was used in bioautography to prevent the diffusion of compounds separated by chromatography or electrophoresis in a seeded agar toward the separation direction. Two main advantages were demonstrated in the present method; (1) better separation and identification of compounds occurring closely positioned, and (2) more sensitive detection owing to compact growth zones. The present method has proved satisfactory and reliable for the routine detection of unknown growth factors.
Journal
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- Japanese Journal of Microbiology
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Japanese Journal of Microbiology 14 (1), 9-13, 1970
Center For Academic Publications Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282681296514048
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- NII Article ID
- 130004237102
- 40018694594
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- NII Book ID
- AA00246855
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- COI
- 1:CAS:528:DyaE3cXltFKqu74%3D
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- NDL BIB ID
- 8511674
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- ISSN
- 00215139
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- PubMed
- 4906559
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed