Comparative Sensitivity of Various Tissue Cultures for Isolation of Coxsackievirus B3

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  • KAWANA Rinji
    Department of Bacteriology, School of Medicine, Iwate Medical University
  • MATSUMOTO Ichiro
    Department of Bacteriology, School of Medicine, Iwate Medical University

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Attempted virus isolation from fecal specimens using four different cell cultures in parallel: primary human embryonic kidney (PHEK) cells, human embryonic lung diploid (DHEL) cells, human cancer HEp-2 cell line and African green monkey kidney VERO cell line. Of 266 rectal swabs obtained from children, ages 0 to 11 from a small town in northern Honshu during August 1966, 74 (27.8%) yielded viral isolates. These included 58 coxsackievirus B3, 7 coxsackievirus B4, 3 echovirus 6, 3 adenoviruses, and 3 unidentified isolates. Of special interest is that practically no clinical disease was associated with this evident outbreak of coxsackievirus B3 infection. Our results provide evidence that HEp-2 and VERO cells are most sensitive, PHEK cells are less sensitive, DHEL cells are the least sensitive for isolation of coxsackievirus B3, isolation rates were 19.2, 12.8, and 7.5%, respectively. Sensitivity differences were revealed by time of appearance and rapidity of progress of the cytopathic effect, and the infectious titer attained in the passages of the isolates.

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