Factors Influencing the Establishment of Persistent Infection of HVJ (Sendai Virus) in L Cells

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  • SUGAMURA Kazuo
    Department of Bacteriology, Tohoku University School of Medicine
  • TOZAWA Hideki
    Department of Bacteriology, Tohoku University School of Medicine Department of Microbiology, Tohoku University School of Dentistry
  • HOMMA Morio
    Department of Bacteriology, Tohoku University School of Medicine Department of Bacteriology, Yamagata University School of Medicine
  • ISHIDA Nakao
    Department of Bacteriology, Tohoku University School of Medicine

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Description

Infection of a subline of L cells adapted to grow in suspension (Ls) with Fushimi strain of HVJ (HVJ-F) resulted in a virus carrier state. Ls cells, when cultured in monolayer, showed morphological changes following infection of HVJ-F and were detached from the glass wall. However, when the detached cells were transferred to a new environment of suspension culture within 5 days after infection, the carrier state was again established. HVJ-F caused only lethal infection in L cells maintained exclusively in monolayer (Lm). On the other hand, both Ls and Lm, irrespective of their culture conditions, were lethally infected by Nagoya 1-60 strain of HVJ. The overall results showed that culture condition as well as the kind of host cells or virus strains is an important factor regulating the establishment and maintenance of the virus carrier state.

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