Complement Fixation Reaction and Agar Gel Double Diffusion Test in Tyzzer's Disease of Mice

DOI Web Site Web Site Web Site PubMed 被引用文献2件 参考文献13件
  • FUJIWARA Kôsaku
    Department of Veterinary Science, Institute for Infectious Diseases, University of Tokyo

この論文をさがす

抄録

Complement fixation reaction and agar gel double diffusion test were made using as antigens, water or phosphate buffer saline extracts of liver from mice severely infected with Tyzzer's disease. The supernatant of the extract heated at 56C for 30 minutes was highly reactive with immune mouse serum in the complement fixation test, while an intense precipitate band was observed only with unheated extracts in agar gel double diffusion tests. Cross reactions were observed in both tests between liver extracts from mice infected with different strains of organisms originating from different sources and showing varied degrees of virulence. Such reactions were not shown between Tyzzerimmune serum and liver extracts from uninfected or murine hepatitis-infected mice, nor were they shown between the Tyzzer-antigen and normal or anti-murine hepatitis virus serum. The complement fixation test was used to test retired breeder mouse sera for disease contamination in mouse colonies. Positive reactions were observed in test sera collected from some breeding colonies that subsequently proved to be contaminated by the cortisone provocation test in young animals.

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (13)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ