Purification of Host DNA Synthesis-Suppressing Factor (DSF) Produced by Infection with Measles Virus

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  • YAMAMOTO Tomoko K.
    Department of Microbiology, Hokkaido University School of Medicine Faculty of Pharmaceutical Science, Chiba University
  • MINAGAWA Tomonori
    Department of Microbiology, Hokkaido University School of Medicine
  • IIDA Hiroo
    Department of Microbiology, Hokkaido University School of Medicine

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  • Purification of host DNA synthesis supp

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Host DNA synthesis-suppressing factor (DSF) produced into culture fluid of cloned HeLa cells (HeLa C-9) infected with a small plaque variant of Toyoshima strain of measles virus was purified by precipitation with ammonium sulfate, chromatography on CM-cellulose and DEAE-cellulose, and gel-filtration on Sephadex G-100 and G-200. The specific activity of the finally purified DSF was 302 units/mg of protein representing approximately 300-fold purification. The molecular weight of DSF was estimated to be about 55000. By isoelectric focusing, two kinds of DSF having isoelectric points of 4.24 and 5.24 were detectable. The purified DSF was able to suppress host DNA synthesis of HeLa cells, continuous human lymphoid cells (NC-37), mouse L cells and Meth-A cells derived from an ascitic tumor of the mouse. The activity of the purified DSF was inactivated by heating at 56 C for 30 min or by treatment with trypsin.

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