Inhibition by β-Carotene and Astaxanthin of NADPH-Dependent Microsomal Phospholipid Peroxidation
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- NAKAGAWA Kiyotaka
- Department of Applied Biological Chemistry, Tohoku University, Sendai 981, Japan
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- KANG Sung-Do
- Department fo Biochemistry, Kon-Kuk University, Choong Joo 380, Korea
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- PARK Dong-Ki
- Department fo Biochemistry, Kon-Kuk University, Choong Joo 380, Korea
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- HANDELMAN Garry J.
- USDA Human Nutrition Research Center on Aging, Tufts University
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- MIYAZAWA Teruo
- Department of Applied Biological Chemistry, Tohoku University, Sendai 981, Japan
書誌事項
- タイトル別名
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- Inhibition by .BETA.-Carotene and Astaxanthin of NADPH-Dependent Microsomal Phospholipid Peroxidation.
- Inhibition by ベーターCarotene and Astaxant
- Inhibition of beta-carotene and astaxanthin of NADPH-dependent microsomal phospholipid peroxidation
この論文をさがす
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To evaluate the antioxidant effects of 48-carotene and asta-xanthin, rat liver microsomes were exposed to a mixture of chelated iron (Fe3+/ADP) and NADPH. The carotenoids (190 pmol/mg protein) were incorporated into some of these microsomal membranes, and phospho-lipid hydroperoxides (PLOOH), thiobarbituric acid reactive substances (TBARS) and endogenous a-tocopherol content were measured over time after the initiation of oxidant stress. In control microsomes, oxidant stress led to accumulation of 1, 865 (+/-371) pmol PLOOH/mg protein during the initial 10-min peroxidation reaction, followed by a more gradual increase during the subsequent 20-min of reaction. PLOOH accumulation during the initial 10-min reaction period was reduced to 588 (+/-169) pmol/mg protein with β-carotene present and 800 (+/-288) pmol/mg protein with astaxanthin present. During the following 20-min of incubation, PLOOH levels declined in the carotenoid-supplemented microsomes but continued to increase at a slower rate in control prepara-tions. TBARS did not show such large accumulation as observed in PLOOH during the initial 10-min incubation in any microsomal sample. The presence of carotenoids in the microsomal membrane partially in-hibited the loss of a-tocopherol, especially during the later phase of oxidant stress. When lipid peroxidation is generated by membrane-bound cyt-P450, the specific measurement of PLOOH clearly demonstrates that the presence of carotenoids provides antioxidant protection.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 43 (3), 345-355, 1997
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詳細情報 詳細情報について
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- CRID
- 1390282681297584000
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- NII論文ID
- 130001370284
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- NII書誌ID
- AA00703822
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- COI
- 1:CAS:528:DyaK2sXksFClsL8%3D
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- ISSN
- 18817742
- 03014800
- http://id.crossref.org/issn/03014800
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- NDL書誌ID
- 4251902
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- PubMed
- 9268922
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可