Studies on Mycobacterium lepraemurium

OGAWA TATSUJI, MOTOMURA KEIKO

doi:10.5025/hansen1930.38.4_246

An aqueous suspension of M. lepraemurium (Hawaiian strain), prepared from the organs of mice previously inoculated with this bacillus before a long period of time, was inoculated intravenously and subcutaneously into the mice of ddN strains. These mice were killed and autopsied from time to time. The removed organs, lymph nodes and subcutaneous nodules were ground in the mortar. The most part of ground materials was inoculated onto the 1% egg yolk medium principally and also onto 3% Ogawa's egg media and 1% Ogawa's egg media. These media were incubated at 37°C for nearly 3 months. The reading was assumed positive when the colony-like products were observed macroscopically.<br>The following was the results: The material which showed most abundant growth of bacilli in the mice during 5-8 months, gives positive result in 14 cases of 22 (63.8%) using 1% egg yolk media, while only one positive case out of 4 using 3% Ogawa's egg media. No positive case was found in 1% Ogawa's egg media. The colony-like products appeared to be a R-Type with somewhat moist nature and showed a very thin yellowish tint. We have succeeded so far in obtaining four successive cultures. The growth was very slow and almost took 2-3 months until the definite growth was assured. A smear specimen displayed well-stained acid-fast bacilli, among them also many elongated ones. These bacilli grew only at 37°C but failed to grow either at 22°C or 45°C. Reproduction test of these bacilli to the mice was favorable. Such a property is identical in every isolated bacilli.

Studies on Mycobacterium lepraemurium

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