Studies on the phospholipid metabolism in choline-deficient guinea pigs-2-Effects of feeding on a N-methylethanolamine-supplemented diet

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  • 小林 正直
    Second Department of Biochemistry, School of Medicine Nagoya University

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  • Studies on the Phospholipid Metabolism in Choline-Deficient Guinea Pigs
  • II. Effects of Feeding on a <i>N</i>-methylethanolamine-Supplemented Diet

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1. Young guinea pigs fed on a choline-deficient diet supplemented with 0.117% MME showed as good growth and survival rate as those fed on a choline-supplemented diet (control). Addition of a larger amount of MME (0.328%) in the diet, however, resulted in a low growth rate and a high mortality.<br>2. In liver slices of MME-fed guinea pigs, much more methyl group of methionine-C3H3 was incorporated into phospholipids than in those of the control ones; one-third of radioactive methyl incorporated was found in phosphatidyl-DME and two-thirds in lecithin. The high values of incorporation and the distribution of radioactivity were not affected by preincubation with MME or DME.<br>3. Liver microsome of MME-fed guinea pigs showed a much higher incorporation of methyl group of S-adenosylmethionine-14CH3 into phospholipids than those of control or choline-deficient ones.<br>4. When the lipids extracted from livers of MME-fed guinea pigs (M-lipid) were added to the reaction system containing liver microsomes from control or choline-deficient animals, the incorporation of labeled methyl group from S-adenosylmethionine-14CH3 was markedly enhanced.<br>5. After addition of M-lipid, liver microsomes from choline-deficient guinea pigs incorporated twice as much methyl group of S-adenosylmethionine-14CH3 into phospholipids than those from control ones.<br>6. It was proved that guinea pig liver microsomes possessed enough activity to carry out the second and the third methylation step of lecithin synthesis from phosphatidylethanolamine, that this was increased in a choline-deficient state, and that a considerable amount of phosphatidyl-MME and -DME were deposited in the livers of MME-fed guinea pigs.

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