Purification and Some Properties of Cobalamin-Dependent Methionine Synthase from Rat Liver.
-
- YAMADA Kazuhiro
- Faculty of Engineering Okayama University Faculty of Education, Okayama University
-
- TOBIMATSU Takamasa
- Faculty of Engineering Okayama University
-
- KAWATA Tetsunori
- Faculty of Agriculture, Tokyo University of Agriculiure Okayama University
-
- WADA Masahiro
- Faculty of Education, Okayama University
-
- MAEKAWA Akio
- Faculty of Education, Okayama University
-
- TORAYA Tetsuo
- Faculty of Engineering Okayama University
書誌事項
- タイトル別名
-
- Purification and Some Properties of Cob
この論文をさがす
説明
Cobalamin-dependent methionine synthase was purified from rat liver. The enzyme activity was separated into two peaks upon Mono-Q column chromatography. Peaks I and II of the enzyme, eluted in this order, were purified 18, 000- and 44, 000-fold in overall yields of 0.7 and 1.8%, respectively. Peak II methionine synthase, the major fraction, was homogeneous as judged by SDS-polyacrylamide gel electrophoresis. The enzyme was a large monomeric protein with an apparent molecular weight of 143, 000 Da. Interconversion of the enzyme between the two peaks was not observed during purification procedures. The enzyme required S adenosylmethionine and a reducing system for activity. Ap-parent Km values of the peak II enzyme for 5-methyltetrahydrofolate and homocysteine were 75 and 1.7μM, respectively.
収録刊行物
-
- Journal of Nutritional Science and Vitaminology
-
Journal of Nutritional Science and Vitaminology 43 (2), 177-186, 1997
一般財団法人 学会誌刊行センター
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681299602688
-
- NII論文ID
- 130001370270
-
- NII書誌ID
- AA00703822
-
- COI
- 1:CAS:528:DyaK2sXjslegsLc%3D
-
- ISSN
- 18817742
- 03014800
- http://id.crossref.org/issn/03014800
-
- NDL書誌ID
- 4231092
-
- PubMed
- 9219091
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可