Simultaneous Determination of α-,γ-Tocopherol and Their Quinones in Rats Plasma and Tissues Using Reversed-phase High-perhrmance Liquid Chromatography
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- KIYOSE Chikako
- Institute of Environmental Science for Human Life, Ochanomizu University
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- SAITO Hisako
- Institute of Environmental Science for Human Life, Ochanomizu University
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- UEDA Tadahiko
- Tokyo Metropolitan Research Laboratory of Public Health
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- IGARASHI Osamu
- Ibaraki Christian University
書誌事項
- タイトル別名
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- Simultaneous Determination of .ALPHA.-, .GAMMA.-Tocopherol and Their Quinones in Rats Plasma and Tissues Using Reversed-phase High-performance Liquid Chromatography.
- Simultaneous Determination of アルファ ガンマ Tocopherol and Their Quinones in Rats Plasma and Tissues Using Reversed phase High perhrmance Liquid Chromatography
この論文をさがす
抄録
We established a method to determine simultaneously α-and γ-tocopherol (-Toc) and their quinones (α-TQ and p-γ-TQ) in biological samples by reverse-phase high-performance liquid chromatography (HPLC). Tocs had a shorter retention time than TOs, and α-forms had a shorter retention times than γ-forms. Four peaks of Tocs and TQs were completely separated by this method. Subsequently, we investigated the distribution of α-, γ-Toc and To in rat tissues and the excretion of Tocs and Tos in rat bile by the above HPLC method. Rats deficient in vitamin E were divided into two groups, γ-Toc group and α+ γ-Toc group, and tissues were collected at 6 and 24 h after intravenous administration of Tocs. Also, bile collection was started immediately and performed at 3 h intervals during 24 h after intravenous administration. The concentration of α-and γ-Toc and their quinones in plasma, tissues and bile were determined by this method. γ-Toc concentration in the liver of α+ γ-Toc group was higher than that of γ-Toc group. However, p-γ-TQ in the liver was not significantly different between α+γ-Toc group and γ-Toc group. Also, both α-TO and p-γ-TQ were present in very low concentrations in all tissues. Therefore, we suggested that the distribution of γ-Toc is affected by a-Toc present in vivo, but the oxidative production of p-γ-TQ from γ-Toc is not affected.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 47 (2), 102-107, 2001
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詳細情報 詳細情報について
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- CRID
- 1390282681300070528
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- NII論文ID
- 10010805401
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- NII書誌ID
- AA00703822
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- COI
- 1:CAS:528:DC%2BD3MXktlKmtb0%3D
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- ISSN
- 18817742
- 03014800
- http://id.crossref.org/issn/03014800
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- NDL書誌ID
- 5796764
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- PubMed
- 11508699
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可