Abnormal Increase in the Expression Level of Proliferating Cell Nuclear Antigen (PCNA) in the Liver and Hepatic Injury in Rats with Dietary Cobalamin Deficiency
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- NAKAO Motoyuki
- Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- KONO Nozomi
- Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- ADACHI Satoko
- Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- EBARA Shuhei
- Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- ADACHI Tetsuya
- Center for Research and Development of Bioresources, Osaka Prefecture University
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- MIURA Takumi
- Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- YAMAJI Ryoichi
- Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- INUI Hiroshi
- Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University Center for Research and Development of Bioresources, Osaka Prefecture University
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- NAKANO Yoshihisa
- Department of Applied Biological Chemistry, Graduate School of Life and Environmental Sciences, Osaka Prefecture University Center for Research and Development of Bioresources, Osaka Prefecture University
Bibliographic Information
- Other Title
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- Abnormal Increase in the Expression Level of Proliferation Cell Nuclear Antigen (PCNA) in the Liver and Hepatic Injury in Rats with Dietary Cobalamin Deficiency
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Abstract
Dietary cobalamin (Cbl; vitamin B12) deficiency resulted in severe growth retardation in rats, and body weight in the Cbl-deficient rats at 20 wk of age was significantly lower compared with the age-matched Cbl-sufficient control rats. In contrast, liver weight, when normalized to body weight, was greater in the Cbl-deficient rats than in the controls (p<0.05). The expression level of proliferating cell nuclear antigen (PCNA), a marker for cell proliferation, in the liver was significantly enhanced in the deficient rats, suggesting that cell proliferation is abnormally activated in the liver under Cbl-deficient conditions. In addition, plasma alanine aminotransferase (ALT) activity, a marker for hepatic injury, was also significantly elevated in the deficient rats. When L-carnitine, which is used clinically for the treatment of Cbl-deficient patients with methylmalonic aciduria, was administered to the Cbl-deficient rats by intraperitoneal injection twice per day for 2 wk (each 0.5 mmol), the amount of methylmalonic acid excreted into the urine was significantly reduced, and the plasma ALT activity was lowered to a normal level. However, the PCNA expression in the liver was barely influenced by the treatment with carnitine. In contrast, when the deficient rats were fed an L-methionine-supplemented diet (4 g of L-methionine per kg of the diet) for 2 wk, the increased expression of PCNA was normalized.
Journal
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 52 (3), 168-173, 2006
Center for Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390282681301487104
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- NII Article ID
- 110004740382
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- NII Book ID
- AA00703822
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- COI
- 1:CAS:528:DC%2BD28XntFehsLg%3D
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- ISSN
- 18817742
- 03014800
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- NDL BIB ID
- 7965026
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed