A Dried Tofu-Supplemented Diet Affects mRNA Expression of Inflammatory Cytokines in Human Blood
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- TOVAR Vivian
- Hitachi Chemical Research Center, Inc.
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- OGURA Mieko
- Hitachi Chemical Research Center, Inc.
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- KOBAYASHI Ai
- Sekino Clinical Pharmacology Clinic
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- KIMURA Sayaka
- Sekino Clinical Pharmacology Clinic
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- SAITO Kazunori
- Sekino Clinical Pharmacology Clinic
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- KAINUMA Michiko
- Sekino Clinical Pharmacology Clinic
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- YOHDA Masafumi
- Department of Biotechnology and Life Sciences, Graduate School of Technology, Tokyo University of Agriculture and Technology
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- HASEGAWA Setsuo
- Sekino Clinical Pharmacology Clinic
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- MITSUHASHI Masato
- Hitachi Chemical Research Center, Inc.
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In order to develop a new model of diet research, blood was drawn from 12 adult volunteers for 3 wk on regular diets as controls, and for a subsequent 3 wk supplemented with 18.5 g of freeze-dried tofu (Koya tofu) every day. Triplicate aliquots of 0.06 mL each of whole blood were stimulated ex vivo with phytohemagglutinin (PHA)-P, heat aggregated human IgG (HAG), lipopolysaccharide (LPS), zymosan A, and anti-T cell receptor (TCR) monoclonal antibody to activate specific subsets of leukocytes, then the levels of various inflammatory cytokine mRNA were quantified by real time PCR. Koya tofu significantly (p<0.05) augmented the fold increase of PHA-induced tumor necrosis factor superfamily (TNFSF) 15, IL6, and IL8, HAG-induced TNFSF15 and IL8, LPS-induced IL6 and IL8, zymosan-induced TNFSF15, IL6 and IL8, and TCR-induced TNFSF2 in comparison to the regular diet. Such increase was due to the reduction of baseline mRNA expression, not the enhancement of mRNA induction after specific stimulations. Six (TNFSF15), 4 (IL6), and 3 (IL10) subjects showed significant reduction of baseline mRNA during the Koya tofu diet compared to that of the control diet. Despite large individual-to-individual and day-to-day variation of mRNA, the method employed in this study was sensitive enough to identify statistically significant results as a group as well as on an individual basis, which will be a foundation for tailored diet in the future. The results also indicated that Koya tofu had a power to alter mRNA expression in leukocytes, and TNFSF15, IL6, and IL10 would be biomarkers for soy.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 56 (6), 396-410, 2010
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詳細情報 詳細情報について
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- CRID
- 1390282681301565056
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- NII論文ID
- 10027415376
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- NII書誌ID
- AA00703822
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- COI
- 1:CAS:528:DC%2BC3MXhtV2qsLg%3D
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- ISSN
- 18817742
- 03014800
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- NDL書誌ID
- 10933053
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可