Determination of Individual Vitamin B<sub>6</sub> Compounds Based on Enzymatic Conversion to 4-Pyridoxolactone
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- NISHIMURA Saki
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
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- NAGANO Sayaka
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
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- A. CRAI Chan
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
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- YOKOCHI Nana
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
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- YOSHIKANE Yu
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
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- GE Fei
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
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- YAGI Toshiharu
- Department of Bioresources Science, Faculty of Agriculture, Kochi University
Bibliographic Information
- Other Title
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- Determination of individual vitamin B6 compounds based on enzymatic conversion to 4-pyridoxolactone
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Description
A determination method for individual natural vitamin B6 compounds was developed. The vitamin B6 compounds were specifically converted into 4-pyridoxolactone (PAL), a highly fluorescent compound, through a combination of enzymatic reactions and HCl-hydrolysis. PAL was then determined by HPLC. Pyridoxal was completely oxidized to PAL with pyridoxal 4-dehydrogenase (PLDH). Pyridoxine and pyridoxamine were totally converted into PAL through a coupling reaction involving pyridoxine 4-oxidase and PLDH, and one involving pyridoxamine-pyruvate aminotransferase and PLDH, respectively. The 5'-phosphate forms and pyridoxine-β-glucoside were hydrolyzed with HCl, and then determined as their free forms. Pyridoxine 5'-phosphate and pyridoxine-β-glucoside were not separately determined here. Three food samples were analyzed by this method.
Journal
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 54 (1), 18-24, 2008
Center for Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390282681301746304
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- NII Article ID
- 110006622656
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- NII Book ID
- AA00703822
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- COI
- 1:CAS:528:DC%2BD1cXjtlKmtr0%3D
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- ISSN
- 18817742
- 03014800
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- NDL BIB ID
- 9399385
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- PubMed
- 18388403
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- JaLC
- IRDB
- NDL Search
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE
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- Abstract License Flag
- Disallowed