Effect of various nucleotides and drugs on microsomal thiamine diphosphatase activity in rat brain.

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  • IWATA Heitaroh
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University
  • MATSUDA Toshio
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University
  • BABA Akemichi
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University

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  • Effect of various uncleotides and drugs on microsomal thiamine diphosphatase activity in rat brain
  • Effect of various uncleotides and drugs

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Abstract

The basic properties of microsomal thiamine diphosphatase (TDPase) in rat brain were examined. It was mainly localized in the microsomal fraction. Microsomal TDPase showed an absolute requirement for divalent cation which was best satisfied by Ca++. Theophylline (1mM), NADP (0.1mM) and NADPH (0.1mM) significantly inhibited its activity. However, caffeine, theobromine, NAD and various putative neurotransmitters did not affect the enzyme activity. UDP was inhibitory and IDP was a competitive inhibitor, but ATP had no effect on the enzyme activity.

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