書誌事項
- タイトル別名
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- Partial Purification and Properties of
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抄録
Four chromatographically different proteases were par-tially purified from defatted soybean flour, and their pH optima were around 5.0 to 5.6 using casein as the substrate. These soybean proteases were designated S1, S2, S3 and S4 according to their order of elution from a DEAE-cellulose column. Each gave a single peak of caseinolytic activity on a Sephadex G-200 column chromatogram, and corresponded to the molecular weights of about 50, 000(S1), 35, 000(S2), 60, 000(S3) and 200, 000(S4). The proteases could hydrolyze casein and poly-Glu. α-Casein was more rapidly hydrolyzed than β-casein, but the esters or dipeptide could not be hydrolyzed. Aliquots of 10-3 M Hg2+, Cu2+ and Zn2+ inhibited the caseinolytic activities by 70% to 90%, while other cations, Mn2+, Mg2+, Ca2+ and Ni2+, at the same concentration did not. SPI (10-5M) inhibited 80-90% of their activities, and EPNP (10-5M) inhibited their activities 30-60%, but DFP (10-3 M), SSI (10-3M), PCMB (10-4M), NEM (10-3M) and EDTA (10-3 M) were not inhibitory. The above results indicate that proteases S1, S2, S3 and S4 from defatted soybean flour can be classified as acid proteases.
収録刊行物
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 25 (4), 333-342, 1979
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詳細情報 詳細情報について
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- CRID
- 1390282681301789696
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- NII論文ID
- 130001371961
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- NII書誌ID
- AA00703822
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- ISSN
- 18817742
- 03014800
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- NDL書誌ID
- 2092479
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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