Determination of Free Monosaccharides and Detection of Sugar Alocohols in Mature Soybean Seeds

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  • 川村 信一郎
    Laboratory of Biological Chemistry, Faculty of Agriculture, Kagawa University Kagawaken Meizen Junior College
  • 長尾 一志
    Laboratory of Biological Chemistry, Faculty of Agriculture, Kagawa University Ootsuka Pharmaceutical Factory, Inc.
  • 笠井 忠
    Laboratory of Biological Chemistry, Faculty of Agriculture, Kagawa University

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  • Determination of free monosaccharides and detection of sugar alcohols in mature soybean seeds.
  • Determination of Free Monosaccharides a

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Although the oligosaccharide contents of soybeans are well documented, the exact values of monosaccharide contents have not been reported. Elaborate methods of preparative paper chromatography together with gas chromatography established the following data for one variety, Kyushu No. 12. The air-dried cotyledon part (admixed with hypocotyls) contained 0.030% glucose and 0.039% fructose. The hull part contained 0.018% galactose, 0.028% glucose, 0.023% fructose, 0.005% arabinose, and 0.002% xylose. Gas chromatograms of trimethyl-silated monosaccharide fractions revealed the existence of minute amounts of sorbitol, arabitol, xylitol, and mannitol in decreasing order (about 0.03% to 0.001% of whole seeds).

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