Effects of dietary protein or amino acids in the perfusion medium on amino acid metabolism in perfused adult rat liver.
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- FUJITA Yoshiaki
- Nutrition Research Laboratory, Tokyo Metropolitan Institute of Gerontology
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- YAMAMOTO Takashi
- Department of Nutrition, School of Medicine, Tokushima University
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- RIKIMARU Toru
- Nutrition Research Laboratory, Tokyo Metropolitan Institute of Gerontology
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- EBISAWA Hidemichi
- Department of Nutrition, School of Medicine, Tokushima University
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- INOUE Goro
- Department of Nutrition, School of Medicine, Tokushima University
Bibliographic Information
- Other Title
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- Effects of Dietary Protein or Amino Aci
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Abstract
To elucidate the response of amino acid metabolism in the liver to dietary protein and plasma amino acids, the livers of adult rats fed on diet containing 10% (control) or 3% (low-protein) egg protein for 3 weeks were perfused for 120 min with amino acid-free medium in Experiment 1 or medium containing an amino acid mixture simulating that in plasma in Experiment 2. During perfusion about 40% of the free amino acids were lost from the liver in Exp. 1, and about 30% in Exp. 2. During this period, in Exp. l the releases of free amino acids and urea into the medium were 140μmol and 2.52mg, respectively, in the control group and 207μmol and 1.10mg, respectively, in the low-protein group. Thus release was greater than decrease in free amino acids in the liver. Essential amino acids, particularly lysine and branched chain amino acids, were released preferentially. The results suggest that the amount of breakdown of liver protein in the two groups was similar, but that the nitrogen was mainly released as free amino acids in the low-protein group, and as urea in the control group. On the contrary, in Exp. 2 the amount of nitrogen released from the liver was comparable to the decrease in amino acids in the liver, and the releases of urea were also less, being 1.83 mg in the control group and 0.54mg in low-protein group. The results show that amino acid metabolism in the liver is greatly affected by the nutritional state of the animal and the amino acid content of the perfusion fluid.
Journal
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 27 (2), 149-163, 1981
Center for Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390282681302430976
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- NII Article ID
- 130001371724
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- NII Book ID
- AA00703822
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- ISSN
- 18817742
- 03014800
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- NDL BIB ID
- 2348111
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- PubMed
- 7310551
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed