Characterization of a Cellular Immunostimulating Peptide from a Soybean Protein Fraction Digested with Peptidase R
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- EGUSA Shintaro
- Interdisciplinary Graduate School of Science and Technology, Shinshu University Marusan-ai Co., Ltd.
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- OTANI Hajime
- Interdisciplinary Graduate School of Science and Technology, Shinshu University
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Description
An immunostimulating glutamine-rich peptide was purified from a soybean protein fraction digested with Peptidase R produced by Rhizopus oryzae (Ro-digest) by a combination of SP-Sepharose column chromatography and reversed-phase high-performance liquid chromatography. The purified peptide was supposed to be located at or near the glutamine-rich region 202 to 222 of the glycinin G4 subunit. The peptide significantly increased the number of CD8+, CD11b+, and CD49b+ cells in C3H/HeN mouse spleen cell cultures, while 2 chemically synthesized glutamine-rich peptides corresponding to residues 202 to 213 (QQQQQQKSHGGR) and residues 214 to 225 (KQGQHQQEEEEE) of the glycinin G4 subunit increased the number of interleukin (IL)-12+CD11b+ cells. The peptide 202-213 also significantly increased the number of CD49b+, IL-2+CD4+, and interferon-γ+CD4+ cells and stimulated the cytotoxic activity of spleen cells toward the human erythroleukemia cell line K562. These results indicate that the glutamine-rich region of the soybean glycinin G4 subunit stimulates the cellular immune system in mouse spleen cell cultures.
Journal
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 55 (5), 428-433, 2009
Center for Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390282681302682496
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- NII Article ID
- 10025972201
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- NII Book ID
- AA00703822
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- COI
- 1:CAS:528:DC%2BD1MXhtlOqur7F
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- ISSN
- 18817742
- 03014800
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- HANDLE
- 10091/10765
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- NDL BIB ID
- 10401525
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- PubMed
- 19926930
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed