Mode of binding of pyridoxal 5'-phosphate in rat liver ornithine aminotransferase.

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  • SANADA Yukihiro
    Department of Enzyme Chemistry, Institute for Enzyme Research, School of Medicine, Tokushima University
  • SHIOTANI Taiichi
    Department of Enzyme Chemistry, Institute for Enzyme Research, School of Medicine, Tokushima University
  • KATUNUMA Nobuhiko
    Department of Enzyme Chemistry, Institute for Enzyme Research, School of Medicine, Tokushima University

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  • Mode of Binding of Pyridoxal 5′-Phosphate in Rat Liver Ornithine Aminotransferase
  • Mode of Binding of Pyridoxal 5 Phosphat

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Abstract

1. Pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate were effective for the association of apo-form II of ornithine amino transferase [EC 2. 6. 1. 13]; whereas other B6 derivatives, such as pyridoxal, pyridoxamine, pyridoxine and pyridoxine 5'-phosphate, had no effect on form II of this apoenzyme.<br> 2. The pyridoxal 5'-phosphate contents of the native enzyme, and reconstituted forms I and II were determined by two different methods to be 1.5 moles, 2.5 moles and 3.3 moles of pyridoxal 5'-phosphate/mole of enzyme, respectively.

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