ハムスター精子の体外での受精能獲得におよぼす排卵産物とグルコースの影響

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  • Effects of the postovulatory oviduct contents and exogeneous glucose on capacitation of hamster spermatozoa in vitro.
  • ハムスター セイシ ノ タイガイ デ ノ ジュセイノウ カクトク ニ オヨボス

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説明

We reported previously that glucose plays an important role in the acrosome reaction of hamster sperm and in fertilization of hamster eggs in vitro by acting synergistically with some component(s) in the postovulatory oviduct contents. The present study was to investigate whether this synergistical action also plays an important role in inducing hamster sperm capacitation in vitro. The basic medium consisted of 123.6mM NaCl, 1.71 mM CaCl2 and 25.07mM NaHCO3 was used for incubation (5% CO2 in air at 37 C) of epididymal spermatozoa at a concentration of 3.1-5.1×106/ml. Spermatozoa in the medium with either 5.56 mM glucose (Exp. II) or the postovulatory oviduct contents (Exp. III) are difficult to induce hyperactivation and the acrosome reaction during incubation for 5 h. However, when the postovulatory oviduct contents or 5.56 mM glucose were supplemented into the sperm suspensions 5 h after incubation, advanced inductions of hyperactivation and acrosome reaction of spermatozoa were observed; the rates of hyperactivated and acrosome reacted spermatozoa at 3h later were 67.1-77.5% and 69.6-86.8%, respectively. The equivalent rates were obtained in sper-matozoa more than 6-7 h after incubation in the medium with both glucose and the postovulatory oviduct contents (Exp. I). Increased rate of sperm penetration was also observed when the postovula-tory oviduct contents or 5.56 mM glucose were supplemented 5 h after incubation of spermatozoa in the medium with latter or former component. These results indicate that partial capacitation may be induced in the simple medium with only the postovulatory oviduct contents or glucose, but that both components are essential for completion of sperm capacitation and induction of hyperactivated motility of spermatozoa.

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